Selected Publications of the Kung Laboratory

3.  Kung, C. (1971)  Genic mutants with altered system of excitation in Paramecium aurelia. Mutagenesis, screening and genetic analysis of the mutants.  Genetics 69: 29–45.

5.  Kung, C. and R. Eckert (1972)  Genetic modification of electric properties in an excitable membrane.  Proc. Natl. Acad. Sci. USA 69: 93–97.

6.  Kung, C. and Y. Naitoh (1973)  Calcium–induced ciliary reversal in the extracted models of "Pawn", a behavioral mutant of Paramecium.  Science 179: 195–196.

8.  Chang, S.Y. and C. Kung (1973)  Temperature–sensitive pawns: Conditional behavioral mutants of Paramecium aurelia.  Science 180: 1197–1199.

15. Kung, C., S.Y. Chang, Y. Satow, J. Van Houten and H. Hansma (1975)  Genetic dissection of behavior in Paramecium.  Science 188: 898–904.

27. Van Houten, J., S.–Y. Chang and C. Kung (1977)  Genetic analyses of "paranoiac" mutants of Paramecium tetraurelia.  Genetics 86: 113–120.

29. Oertel, D., S.J. Schein and C. Kung (1977)  Separation of membrane currents using a Paramecium mutant.   Nature 268: 120–124.

35. Omoto, C.K. and C. Kung (1979)  The pair of central tubules rotates during ciliary beat in Paramecium.  Nature 279: 532–534.

46. Kung, C. and Y. Saimi (1982)  The physiological basis of taxes in Paramecium.  Ann. Rev. Physiol. 44: 519–534.

48. Saimi, Y. and C. Kung (1982)  Are ions involved in the gating of calcium channels? Science 218: 153–156.

58. Ehrlich, B.E., A. Finkelstein, M. Forte and C. Kung (1984)  Voltage–dependent calcium channels from Paramecium cilia incorporated into planar lipid bilayers.  Science 225: 427–428.

61. Hinrichsen, R.D., Y. Saimi and C. Kung (1984)  Mutants with altered Ca²⁺–channel properties in Paramecium tetraurelia: Isolation, characterization and genetic analysis.  Genetics 108: 545–558.

62. Haga, N., M. Forte, R. Ramanathan, T. Hennessey, M. Takahashi and C. Kung (1984)  Characterization and purification of a soluble protein controlling Ca–channel activity in Paramecium.  Cell 39: 71–78.

67. Hinrichsen, R.D., E. Amberger, Y. Saimi, A. Burgess–Cassler and C. Kung (1985) Genetics of mutants with a reduced Ca²⁺–dependent K⁺ current in Paramecium tetraurelia. Genetics 111: 433–445.

70. Hinrichsen, R.D., A. Burgess–Cassler, B.C. Soltvedt, T. Hennessey and C. Kung (1986)  Restoration of  calmodulin of a Ca²⁺–dependent K⁺ current missing in a mutant of Paramecium.  Science 232: 503–506.

72. Gustin, M.C., B Martinac, Y. Saimi, M.R. Culbertson and C. Kung (1986)  Ion channels in yeast.  Science 233: 1195–1197.

77. Saimi, Y. and C. Kung (1987)  Behavioral genetics of Paramecium.  Ann. Rev. Genetics 21: 47–65.

82. Gustin, M.C., X.–L. Zhou, B. Martinac and C. Kung (1988)  A mechanosensitive ion channel in the yeast plasma membrane.  Science 242: 762–765.

87. Delcour, A.H., B. Martinac, J. Adler and C. Kung (1989)  Modified reconstitution method used in patch–clamp studies of Escherichia coli ion channels.  Biophysical Journal 56: 631–636.

96. Kink, J.R., M.E. Maley, R.R. Preston, K.–Y. Ling, M.A. Wallen–Friedman, Y. Saimi and C. Kung (1990)  Mutations in Paramecium calmodulin indicate functional differences between the C–terminal and the N–terminal lobes in vivo.  Cell 62: 165–174.

98. Martinac, B., J. Adler and C. Kung  (1990)  Mechanosensitive ion channels of E. coli activated by amphipaths.  Nature 348: 261–263.

99. Preston, R.R., J.A. Kink, R.D. Hinrichsen, Y. Saimi and C. Kung (1991) Calmodulin mutants and Ca²⁺–dependent channels in Paramecium.  Ann. Rev. Physiol. 53: 309–319

102. Zhou, X.–L., M.A. Stumpf, H.C. Hoch and C. Kung (1991) A mechanosensitive channel in whole cells and in membrane patches of the fungus Uromyces.  Science 253: 1415–1417.

103. Kanabrocki, J.A., Y. Saimi, R.R. Preston, W.J. Haynes and C. Kung (1991) Efficient transformation of cam2, a behavioral mutant of Paramecium tetraurelia with the calmodulin gene.  Proc. Natl. Acad. Sci. USA 88: 10845–10849.

111. Preston, R.R., Y. Saimi and C. Kung (1992) Calcium current activated upon hyperpolarization of Paramecium tetraurelia.  J. Gen. Physiol. 100: 233–251.

119. Sukharev, S.I., P. Blount, B. Martinac, F.R. Blattner and C. Kung (1994) A large conductance mechanosensitive channel in E. coli encoded by mscL alone.  Nature 368: 265-268.

128. Saimi, Y. and C. Kung (1994) Ion channel regulatiaon by calmodulin binding. FEBS Lett. 350:155-158.

142. Sukharev, S.I., P. Blount, B. Martinac and C. Kung (1997) Mechanosensitive channels of Escherichia coli: The MscL Gene, Protein, and Activities.  Annual Review of Physiology  59: 633-657.

146. Haynes, W.J., B. Vaillant, R.R. Preston, Y. Saimi and C. Kung (1998) The cloning by complementation of the pawn-A gene in Paramecium.  Genetics 149: 947-957.

149. Ou, X., P. Blount, R.J. Hoffman and C. Kung (1998) One face of a transmembrane helix is crucial in mechanosensitive channel gating.  Proc. Natl. Acad. Sci. USA 95: 11471-11475.

156. Yoshimura, K. A. Batiza, M. Schroeder, P. Blount and C. Kung (1999) Hydrophilicity of a single residue within MscL correlates with increased channel mechanosensitivity.  Biophysical J. 77: 1960-72.

160. Haynes, W.J., K.-Y. Ling, R.R. Preston, Y. Saimi and C. Kung (2000) The cloning and molecular analysis of pawn-B in Paramecium tetraurelia.  Genetics 155: 1105-1117.

161. Yoshimura, K., A. Batiza and C. Kung (2001) Chemically charging the pore constriction opens the mechanosensitive channel MscL.  Biophyscial J. 80: 2198-2206.

162. Dessen, P., M.  Zagulski, R. Gromadka, et al. (18 authors) (2001) Paramecium genome survey: a pilot project.  Trends in Genet. 17: 306-308.

163. Palmer, C.P., X.-L. Zhou, J. Lin, S.K. Loukin, C. Kung and Y. Saimi (2001) A TRP homolog in Saccharomyces cerevisiae forms an intracellular Ca²⁺-permeable channel in the yeast vacuolar membrane. Proc. Natl. Acad. Sci. 98: 7801-7805.

164. Ling, K.-Y., W. J. Haynes, L. Oesterle, C. Kung, R. Preston, and Y. Saimi (2001) K⁺-channel transgenes reduce K⁺ currents in Paramecium likely by a post-translational mechanism.  Genetics 159: 987-995.

165. Saimi, Y. and C. Kung (2002) Calmodulin as an ion-channel subunit.  Ann Rev. Physiology 64: 289-311.

166. Batiza, A.F., M.-C. Kuo, K. Yoshimura, and C. Kung (2002) Gating the bacterial mechanosensitive channel MscL in vivo. Proc. Natl. Acad. Sci. USA 99: 5643-48

167. Haynes, W.J., C. Kung, Y. Saimi, R.R. Preston (2002) An exchanger-like protein underlies the large Mg²⁺ current in Paramecium.  Proc. Natl. Acad. Sci. USA 99: 15717-22.

168. Zhou, X.-L., A.F. Batiza, S.H. Loukin, C.P. Palmer, C. Kung and Y. Saimi (2003) The transient receptor potential chanel on the yeast vacuole is mechanosensitive.  Proc. Natl. Acad. Sci. USA 100: 7105-7110.

169. Haynes, W.J., K.-Y. Ling, Y. Saimi, and C. Kung (2003) The PAK paradox: Paramecium appears to have more K⁺-channel genes than humans.  Eukaryot. Cell  2: 737-745.

170. Kuo, M.M.-C., Y. Saimi, and C. Kung (2003)  Gain-of-function mutations indicate that E. coli Kch forms a functional K⁺ conduit in vivo.  EMBO J. 22: 4049-4058.

171. C. Kung and P. Blount (2004) Channels in microbes: so may holes to fill.  Mol.  Microbiol. 53: 373-380.

173. Kuo, M. M.-C. C. Kung, and Y. Saimi (2005)  K⁺ channels: a survey and a case study of Kch of Escherichia coli. In Bacterial Ion Channels and Their Eukaryotic Homologs.  A. Kybalski and B. Martinac (eds.) ASM Press, Washington, D.C. pp. 1-20.

174. Zhou, X.L., S.H. Loukin, R. Coria, C. Kung, and Y. Saimi (2005)  Heterologously expressed fungal transient receptor potential channels retain mechanosensitivity in vitro and osmotic response in vivo.  Eu. Biophysics J. 34: 413-422.

175. Loukin, S.H., M. M.-C. Kuo, X.-L. Zhou, W.J. Haynes, C. Kung, and Y. Saimi (2005)     Microbial K⁺ Channels.  Journal of General Physiology  125: 521-527.

176. Kuo, M. M,-C., W.J. Haynes, S.H. Loukin, C. Kung, and Y. Saimi (2005)  Prokaryotic K⁺ channels: from crystal structures to diversity.  FEMS Microbiology Reviews.  29: 145-170.

177. Anishkin, A. and C. Kung (2005)  Microbial Mechanosensation.  Current Opinion in Neurobiology  15: 397-405.

178. Kung, C. (2005) A possible unifying principle for mechanosensation. Nature  436: 647-     654.

180. Saimi, Y., X.-L. Zhou, S.H. Loukin, W.J. Haynes and C. Kung (2007) Microbial TRP channels and their mechanosensitivity. in Mechanosensitive Ion Channels, Ed. O. Hamill Current Topics in Membranes, vol, 58, Elsevier Press. pp. 311-327.

181. Loukin, S., C. Kung, and Y, Saimi (2007) Lipid perturbations sensitize osmotic down-shock activated Ca2+ influx, a yeast "deletome" analysis.  FASEB J. 21: 1813-1820.

183. Zhou, X.-L., Z.-W. Su, A Anishkin, W.J. Haynes, E.M. Friske, S.H. Loukin, C. Kung, and Y. Saimi (2007) Yeast screens show aromatic residues at the end of the sixth helix anchor TRP-channel gate.  Proc. Natl. Acad. Sci. 104: 15555-15559.

184. Kung, C., X.-L. Zhou, Z.-W. Su, W.J. Haynes, S.H. Loukin and Y. Saimi (2007)  Microbial senses and ion channels, in Sensing with Ion Channels.  Ed. B. Martinac, Springer-Verlag, pp. 1-23.

185. Su, Z., X.-L. Zhou, W.J. Haynes, S.H. Loukin, A. Anishkin, Y. Saimi and C. Kung (2007)  Yeast gain- of-function mutations reveal structure-function relationships conserved among different subfamilies of transient receptor potential channels.  Proc. Natl. Acad. Sci. 104: 19607-1961.

186. Loukin, S.,X.-L. Zhou C. Kung, and Y, Saimi (2008) A Genome-wide survey suggests an osmo-protective sole for vacuolar Ca2+ release in cell-wall-compromised yeast.  FASEB J.  22: 2405-2415.

187. Haynes, W.J., X.-L. Zhou, Z-W. Su, S.H. Loukin, Y. Saimi, and C. Kung (2008) Indole bypasses the usual gating principles to activate the yeast TRPY1 channel. FEBS Letters 582: 1514.

188. Martinac, B., Y, Saimi. and C. Kung (2008) Ion channels in microbes.  Phys. Reviews 88: 1449-1490.                                                                

189. Myers, B.R., Y. Saimi, D. Julius, and C. Kung (2008) Multiple unbiased prospective screens identify TRP channels and their conserved gating elements.  J. Gen. Physiol. 132: 481-486. 

190. Loukin, S.H., Z.-W. Su, and C. Kung (2009)  Hypotonic shock activates rat TRPV4 in yeast in the absence of polyunsaturated fatty acids. FEBS Lett. 583: 754-758.

192.      Su, Z.-W., X.-L. Zhou, S.H. Loukin, W.J. Haynes, Y. Saimi, and C. Kung (2009) The use of yeast to understand TRP-channel mechanosensitivity  Pflugers Arch. Eur. J. Physiol. 458: 861-867.

193. Loukin, S., Z.-W. Su, X.-L. Zhou, and C. Kung (2010) Forward genetic analysis reveals multiple gating mechanisms of TRPV4. J. Biol. Chem. 285: 19884-19890.

194. Loukin, S., X.-L. Zhou, Z.-W. Su, Y. Saimi, and C. Kung (2010) Wild-type and brachyolmia-causing mutant TRPV4 channels respond directly to stretch force. J. Biol. Chem. 285: 27176-27181.

195. Kung, C., B. Martinac, and S. Sukharev (2010) Mechanosensitive channels in microbes. Annu. Rev. Miccrobiol. 64: 313-329.

196. Loukin, S., Z.-W. Su, and C. Kung (2011) Increased basal activity is a key determinant in the severity of human skeletal dysplasia caused by TRPV4 mutations. PLoS ONE 6(5): e19533.

197. Su, Z.-W., A. Anishkin, C. Kung, and Y. Saimi (2011) The core domain as the force sensor of the yeast mechanosensitive TRP channel. J. Gen. Physiol. 138: 627-640.

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