Selected Publications of the Kung Laboratory

3.  Kung, C. (1971)  Genic mutants with altered system of excitation in Paramecium aurelia. Mutagenesis, screening and genetic analysis of the mutants.  Genetics 69:29–45.

5.  Kung, C. and R. Eckert (1972)  Genetic modification of electric properties in an excitable membrane.  Proc. Natl. Acad. Sci. USA69:93–97.

6.  Kung, C. and Y. Naitoh (1973)  Calcium–induced ciliary reversal in the extracted models of "Pawn", a behavioral mutant of Paramecium.  Science 179:195–196.

8.  Chang, S.Y. and C. Kung (1973)  Temperature–sensitive pawns: Conditional behavioral mutants of Paramecium aurelia.  Science 180:1197–1199.

15. Kung, C., S.Y. Chang, Y. Satow, J. Van Houten and H. Hansma (1975)  Genetic dissection of behavior in Paramecium.  Science 188:898–904.

27. Van Houten, J., S.–Y. Chang and C. Kung (1977)  Genetic analyses of "paranoiac" mutants of Paramecium tetraurelia.  Genetics 86:113–120.

29. Oertel, D., S.J. Schein and C. Kung (1977)  Separation of membrane currents using a Paramecium mutant.   Nature 268:120–124.

35. Omoto, C.K. and C. Kung (1979)  The pair of central tubules rotates during ciliary beat in Paramecium.  Nature 279:532–534.

46. Kung, C. and Y. Saimi (1982)  The physiological basis of taxes in Paramecium.  Ann. Rev. Physiol. 44:519–534.

48. Saimi, Y. and C. Kung (1982)  Are ions involved in the gating of calcium channels?              Science 218:153–156.

58. Ehrlich, B.E., A. Finkelstein, M. Forte and C. Kung (1984)  Voltage–dependent calcium channels from Paramecium cilia incorporated into planar lipid bilayers.  Science 225:427–428.

61. Hinrichsen, R.D., Y. Saimi and C. Kung (1984)  Mutants with altered Ca²⁺–channel properties in Paramecium tetraurelia: Isolation, characterization and genetic analysis.  Genetics 108:545–558.

62. Haga, N., M. Forte, R. Ramanathan, T. Hennessey, M. Takahashi and C. Kung (1984)  Characterization and purification of a soluble protein controlling Ca–channel activity in Paramecium.  Cell 39:71–78.

67. Hinrichsen, R.D., E. Amberger, Y. Saimi, A. Burgess–Cassler and C. Kung (1985)              Genetics of mutants with a reduced Ca²⁺–dependent K⁺ current in Paramecium tetraurelia.        Genetics 111:433–445.

70. Hinrichsen, R.D., A. Burgess–Cassler, B.C. Soltvedt, T. Hennessey and C. Kung (1986)  Restoration of  calmodulin of a Ca²⁺–dependent K⁺ current missing in a mutant of Paramecium.  Science 232:503–506.

72. Gustin, M.C., B Martinac, Y. Saimi, M.R. Culbertson and C. Kung (1986)  Ion channels in yeast.  Science 233:1195–1197.

74. Schaefer, W.H., R.H. Hinrichsen, A. Burgess–Cassler, C Kung, I.A. Blair and D.M. Watterson (1987)  A mutant Paramecium with a defective calcium–dependent potassium conductance has an altered calmodulin:  a nonlethal selective alteration in calmodulin regulation.  Proc. Natl. Acad. Sci. USA 84:3931–3935.

77. Saimi, Y. and C. Kung (1987)  Behavioral genetics of Paramecium.  Ann. Rev. Genetics 21:47–65.

82. Gustin, M.C., X.–L. Zhou, B. Martinac and C. Kung (1988)  A mechanosensitive ion channel in the yeast plasma membrane.  Science 242:762–765.

87. Delcour, A.H., B. Martinac, J. Adler and C. Kung (1989)  Modified reconstitution method used in patch–clamp studies of Escherichia coli ion channels.  Biophysical Journal 56:631–636.

96. Kink, J.R., M.E. Maley, R.R. Preston, K.–Y. Ling, M.A. Wallen–Friedman, Y. Saimi and C. Kung (1990)  Mutations in Paramecium calmodulin indicate functional differences between the C–terminal and the N–terminal lobes in vivo.  Cell 62:165–174.

98. Martinac, B., J. Adler and C. Kung  (1990)  Mechanosensitive ion channels of E. coli activated by amphipaths.  Nature 348:261–263.

99. Preston, R.R., J.A. Kink, R.D. Hinrichsen, Y. Saimi and C. Kung (1991) Calmodulin mutants and Ca²⁺–dependent channels in Paramecium.  Ann. Rev. Physiol. 53:309–319

102. Zhou, X.–L., M.A. Stumpf, H.C. Hoch and C. Kung (1991) A mechanosensitive channel in whole cells and in membrane patches of the fungus Uromyces.  Science 253:1415–1417.

103. Kanabrocki, J.A., Y. Saimi, R.R. Preston, W.J. Haynes and C. Kung (1991) Efficient transformation of cam2, a behavioral mutant of Paramecium tetraurelia with the calmodulin gene.  Proc. Natl. Acad. Sci. USA 88:10845–10849.

111. Preston, R.R., Y. Saimi and C. Kung (1992) Calcium current activated upon hyperpolarization of Paramecium tetraurelia.  J. Gen. Physiol. 100:233–251.

119. Sukharev, S.I., P. Blount, B. Martinac, F.R. Blattner and C. Kung (1994) A large conductance mechanosensitive channel in E. coli encoded by mscL alone.  Nature 368:265-268.

128. Saimi, Y. and C. Kung (1994) Ion channel regulatiaon by calmodulin binding. FEBS Lett. 350:155-158.

142. Sukharev, S.I., P. Blount, B. Martinac and C. Kung (1997) Mechanosensitive channels of Escherichia coli: The MscL Gene, Protein, and Activities.  Annual Review of Physiology  59:633-657.

146. Haynes, W.J., B. Vaillant, R.R. Preston, Y. Saimi and C. Kung (1998) The cloning by complementation of the pawn-A gene in Paramecium.  Genetics 149:947-957.

149. Ou, X., P. Blount, R.J. Hoffman and C. Kung (1998) One face of a transmembrane helix is crucial in mechanosensitive channel gating.  Proc. Natl. Acad. Sci. USA95:11471-11475.

156. Yoshimura, K. A. Batiza, M. Schroeder, P. Blount and C. Kung (1999) Hydrophilicity of a single residue within MscL correlates with increased channel mechanosensitivity.  Biophysical J. 77: 1960-72.

160. Haynes, W.J., K.-Y. Ling, R.R. Preston, Y. Saimi and C. Kung (2000) The cloning and molecular analysis of pawn-B in Paramecium tetraurelia.  Genetics 155: 1105-1117.

161. Yoshimura, K., A. Batiza and C. Kung (2001) Chemically charging the pore constriction opens the mechanosensitive channel MscL.  Biophyscial J. 80: 2198-2206.

162. Dessen, P., M.  Zagulski, R. Gromadka, et al. (18 authors) (2001) Paramecium genome survey: a pilot project.  Trends in Genet. 17: 306-308.

163. Palmer, C.P., X.-L. Zhou, J. Lin, S.K. Loukin, C. Kung and Y. Saimi (2001) A TRP homolog in Saccharomyces cerevisiae forms an intracellular Ca²⁺-permeable channel in the yeast vacuolar membrane. Proc. Natl. Acad. Sci. 98: 7801-7805.

164. Ling, K.-Y., W. J. Haynes, L. Oesterle, C. Kung, R. Preston, and Y. Saimi (2001) K⁺-channel transgenes reduce K⁺ currents in Paramecium likely by a post-translational mechanism.  Genetics 159:987-995.

165. Saimi, Y. and C. Kung (2002) Calmodulin as an ion-channel subunit.  Ann Rev. Physiology 64:289-311.

166. Batiza, A.F., M.-C. Kuo, K. Yoshimura, and C. Kung (2002) Gating the bacterial mechanosensitive channel MscL in vivo. Proc. Natl. Acad. Sci. USA 99:5643-48

167. Haynes, W.J., C. Kung, Y. Saimi, R.R. Preston (2002) An exchanger-like protein underlies the large Mg²⁺ current in Paramecium.  Proc. Natl. Acad. Sci. USA 99 15717-22.

168. Zhou, X.-L., A.F. Batiza, S.H. Loukin, C.P. Palmer, C. Kung and Y. Saimi (2003) The transient receptor potential chanel on the yeast vacuole is mechanosensitive.  Proc. Natl. Acad. Sci. USA 100: 7105-7110.

169. Haynes, W.J., K.-Y. Ling, Y. Saimi, and C. Kung (2003) The PAK paradox: Paramecium appears to have more K⁺-channel genes than humans.  Eukaryot. Cell  2: 737-745.

170. Kuo, M.M.-C., Y. Saimi, and C. Kung (2003)  Gain-of-function mutations indicate that E. coli Kch forms a functional K⁺ conduit in vivo.  EMBO J.22: 4049-4058.

171. C. Kung and P. Blount (2004) Channels in microbes: so may holes to fill.  Mol.  Microbiol. 53: 373-380.

172. Palmer, C.P., A. Batiza, X.-L. Zhou, S.H. Loukin, Y. Saimi and c. Kung (2004)  Ion channels of microbes.  In Cell Signalling in Prokayrotes and Lower Metazoa, I. Fairweather (ed.) Kluwer Academic Publishers, pp. 325-345.

173. Kuo, M. M.-C. C. Kung, and Y. Saimi (2005)  K⁺ channels: a survey and a case study of Kch of Escherichia coli. In Bacterial Ion Channels and Their Eukaryotic Homologs.  A. Kybalski and B. Martinac (eds.) ASM Press, Washington, D.C. pp. 1-20.

174. Zhou, X.L., S.H. Loukin, R. Coria, C. Kung, and Y. Saimi (2005)  Heterologously expressed fungal transient receptor potential channels retain mechanosensitivity in vitro and osmotic response in vivo.  Eu. Biophysics J. 34: 413-422.

175. Loukin, S.H., M. M.-C. Kuo, X.-L. Zhou, W.J. Haynes, C. Kung, and Y. Saimi (2005)     Microbial K⁺ Channels.  Journal of General Physiology  125: 521-527.

176. Kuo, M. M,-C., W.J. Haynes, S.H. Loukin, C. Kung, and Y. Saimi (2005)  Prokaryotic K⁺ channels: from crystal structures to diversity.  FEMS Microbiology Reviews.  29: 145-170.

177. Anishkin, A. and C. Kung (2005)  Microbial Mechanosensation.  Current Opinion in Neurobiology  15: 397-405

178. Kung, C. (2005) A possible unifying principle for mechanosensation.   Nature  436: 647-     654.

179. Kou, M.-C., Y. Saimi, C. Kung and S. Choe (2007) Patch clamp and phenotypic analyses of a prokaryotic cyclic nucleotide-gated K+ channel using Escherichia coli as a hose. Journal of Biological Chemistry 282: 24294-24301.

180. Zhou, X.-L., Su, Z.-W., Anishkin, A., Haynes, W. J., Friske, E. M., Loukin, S. H., Kung, C., and Saimi, Y. (2007) Yeast screens show aromatic residues at the end of the sixth helix anchor TRP-channel gate. Proc. Natl. Acad. Sci. (in press).

.